Lab Protocols

Download our biotechnology lab protocols for high school and college students.

 

This lab provides an introduction to DNA (deoxyribonucleic acid) and the field of biotechnology.

 

DNA Extraction from Strawberry

 

Micropipets are used in almost all biotechnology experiments. These precise instruments allow for the dispensing of extremely small volumes. This lab will help your students become familiar with the instruments.

 

Micropipet Technique

 

In this experiment, students use agarose gel electrophoresis to separate several dyes - a precursor to DNA separations. The lab will help students gain familiarity with equipment and techniques essential to biotechnology.

 

Gel Electrophoresis of Dyes

 

Restriction mapping is a cornerstone of modern-day biotechnology. Restriction enzymes cleave DNA at known sites, making possible detailed analysis of specific sections of an organism’s genome. In this lab, students digest samples of lambda DNA with three different restriction enzymes and one unknown.

 

Restriction Enzyme Analysis

 

In this lab activity, students cause a wild type strain of E. coli to incorporate and express a plasmid containing a gene that codes for a green fluorescent protein (GFP).

 

Bacterial Transformation with Green Fluorescent Protein

 

During this laboratory, students extract their own DNA from a cell sample taken from the lining of their mouth. They then use a powerful molecular biology technique, called the Polymerase Chain Reaction, to amplify this Alu region so that it can be visualized using gel electrophoresis.

 

DNA Amplification of an Alu Insert

 

 

Additional Protocols

Disease Epidemic - ELISA Technique
Microbe Laboratories

 

 

 

Last Updated on Tuesday, 02 November 2010 19:05